Team:Warsaw/Calendar-Main/13 September 2009

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Assembly of endosomal detection operon

Marcin

Task 1

  • Prepare bacterial cultures for isolation of following constructs:
    • BBa_K177037 on pSB1A3 plasmid
    • mitochondrial signal peptide from COX CDS on pKSII plasmid

Task 2

  • Isolation of plasmid from previously prepared cultures

Methods:

  • Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
  • When isolation was done samples were frozen




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