Team:Warsaw/Calendar-Main/13 September 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1
- Prepare bacterial cultures for isolation of following constructs:
- BBa_K177037 on pSB1A3 plasmid
- mitochondrial signal peptide from COX CDS on pKSII plasmid
Task 2
- Isolation of plasmid from previously prepared cultures
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
- When isolation was done samples were frozen
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