Team:Warsaw/Calendar-Main/16 August 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli strain TOP10 to reveal the quality of prepared bacteria
Comment
The quality of this bacteria is superior to previously prepared ones
Task 2:
- Transformation of chemocompetent E. coli strain TOP10
Constructs to transform:
Methods:
- Ligation prepared in 15.08.09 was stopped via thermal inactivation in 80°C for 20 minutes
- Detailed protocol of transformation is described here.
Task 3:
- Isolate backbone plasmid pSB1A3
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
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