Team:Warsaw/Calendar-Main/18 June 2009
From 2009.igem.org
Testing of BioBricks from 2009 distribution and pVDL9 plasmid
Monika, Marek
- Isolation of plasmid DNA from 3ml of overnight bacterial cultures with Plasmid Mini kit (A&A Biotechnology)
- Restriction digest of minipreps with EcoRI and PstI (ca. 200ng of DNA, 0.3ul (3U) of each enzyme, 2ul of Orange buffer, H2O to final volume of 20ul) for 2h.
- Electrophoresis of digests in 1% agarose gel
Isolated DNA | |
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Part/Plasmid | concentration |
B0010 | 118ng/ul |
I14032 | 20ng/ul |
E1010 | 59ng/ul |
R0080 | 25ng/ul |
pVDL9 | 43ng/ul |
(place for the photo of the gel)
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