Team:Warsaw/Calendar-Main/21 August 2009

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Assembly of endosomal detection operon

Marcin


Task 1:

  • Prepare the bacterial cultures for isolation of following constructs:

Digest of isolated plasmids and transformation of chemocompetent E. coli strain DH5&alpha with BBa_C0040 + BBa_R0080 on pSB1A3 were done by Kuba

Making of the plac-RBS-llo part

Jarek


Tasks:

  • Separation of digestion products from 20 August in 0,8% agarose gel.
  • Digestion of 8 plasmid DNA samples with XbaI and PstI endonucleases.
  • Separation of digestion products in 0,8% agarose gel.
  • Transformation competent ''E.coli'' strain with DNA sample containing plac+RBS+llo part and plating the transformated culture on the LB+Amp plates.

Cloning switch 1 regulatory parts [ K177012-PcI.RBS.LacI, K177033-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011-PLacI.RBS.cI.terminator, K177038-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance

Ania


Tasks:







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