Team:Warsaw/Calendar-Main/21 August 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Prepare the bacterial cultures for isolation of following constructs:
Digest of isolated plasmids and transformation of chemocompetent E. coli strain DH5&alpha with BBa_C0040 + BBa_R0080 on pSB1A3 were done by Kuba
Making of the plac-RBS-llo part
Jarek
Tasks:
- Separation of digestion products from 20 August in 0,8% agarose gel.
- Digestion of 8 plasmid DNA samples with XbaI and PstI endonucleases.
- Separation of digestion products in 0,8% agarose gel.
- Transformation competent ''E.coli'' strain with DNA sample containing plac+RBS+llo part and plating the transformated culture on the LB+Amp plates.
Ania
Tasks:
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