Team:Warsaw/Calendar-Main/26 July 2009

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Assembly of endosomal detection operon

Marcin


Task 1:

  • Transformation of chemocompetent E. coli strain DH5α

Methods:

  • Detailed protocol of transformation is described here. The only modification is usage of total volume of ligation mixture prepared 20.07.09
  • petri dish were hold in 37°C for 16 hours

Construction of K177012 operon1_part2

Ania


Tasks:

  • Set up liquid cultures from previously transformed cells to plasmid DNA extraction.
  • Transform cells with repeated RBs.3LacI ligation.

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