Team:Warsaw/Calendar-Main/26 July 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Transformation of chemocompetent E. coli strain DH5α
Methods:
- Detailed protocol of transformation is described here. The only modification is usage of total volume of ligation mixture prepared 20.07.09
- petri dish were hold in 37°C for 16 hours
Construction of K177012 operon1_part2
Ania
Tasks:
- Set up liquid cultures from previously transformed cells to plasmid DNA extraction.
- Transform cells with repeated RBs.3LacI ligation.
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|