Preparation of biobricks to send

Marcin

Task 1: Isolation of plasmid containing BBa_K177016

Methods:

• Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here

Comment:

For better yield of ligations I decided to purificate both insert and vector DNA molecules in one probe.

Task 2: Gel-outs of followed biobricks:

• BBa_K177035 with BBa_K177036
• BBa_K177035 with BBa_K177043

Methods:

All gel-outs were performed using the A&A kit according to the manual