Team:Warsaw/Calendar-Main/28 May 2009

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Cloning of hly gene into pKSII+ vector

Marcin


  • Task:

Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation

Methods:

  • Composition of reaction mixture:
    1 μl purified plasmid solution
    0.5 μl HindIII (Fermentas)
    2 μl Buffer Red (Fermentas)
    15.5 μl MQ water
  • Digest

Program:

description of the program is here

After digest samples were loaded into gel and electrophoretically separated


Electrophoresis conditions:

voltage - 100V

time: 40 minutes



Fig.1. Restriction pattern of analysed samples

Comment:

Sample 1 and 2 have expected resctricion pattern.





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