Team:Warsaw/Calendar-Main/28 May 2009
From 2009.igem.org
Cloning of hly gene into pKSII+ vector
Marcin
- Task:
Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation
Methods:
- Composition of reaction mixture:
1 μl purified plasmid solution 0.5 μl HindIII (Fermentas) 2 μl Buffer Red (Fermentas) 15.5 μl MQ water
- Digest
Program:
description of the program is here
After digest samples were loaded into gel and electrophoretically separated
Electrophoresis conditions:
voltage - 100V
time: 40 minutes
Fig.1. Restriction pattern of analysed samples
Comment:
Sample 1 and 2 have expected resctricion pattern.
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