Team:Warsaw/Calendar-Main/2 July 2009
From 2009.igem.org
Cloning of mitochondrial targeting signal
Sebastian
Tasks:
- Isolation of the pAcGFP1-Mito plasmid (containing mitochondrial targeting signal from subunit VII ot the human cytochrome C oxidase (Cox))
Methods:
Isolation of plasmid DNA from 1,5ml of overnight bacterial culture with Plasmid Mini kit (A&A Biotechnology). Detailed procedure of the isolation is described here
Plasmid concentration in isolated sample - 50ng/ul
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