Team:Warsaw/Calendar-Main/2 October 2009
From 2009.igem.org
cloning of cro (another shot)
Kuba
Task: Obtain a brick vector carrying the cro gene, with a full, correct prefix and suffic
Method used:
- digest of pSB and cro PCR product with XbaI and SpeI
- ligation
- transformation
- isolation
- control digest with XbaI (in the inverse orientation, the insert eliminates this restriction site)
time elapsed: 3 days
results: the brick was succesfully obtained
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