Team:Warsaw/Calendar-Main/2 September 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Restriction digest of followed costruct: BBa_K177037 from the pSB1A3 plasmid
Methods:
- Reaction mixture composition:
1. μl purified plasmid DNA product 1 μl XbaI (Fermentas) 1 μl PstI (Fermentas) 2 μl Buffer Tango (Fermentas) 15 μl MQ water
Results
- All isolated plasmids have no insert
Task 2:
- Prepare the bacterial cultures for isolation of on pSB1A3 plasmid containing BBa_K177035
Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Preparation of PCR reaction with L.monocytogenes st. EGD-e genome DNA
- Separation of PCR products on the 0,8% agarose gel
- Acquiring the pure DNA sample from PCR reaction with Clean-Up kit
Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (both of them) were transferred to a new plate containing ampicilin
- Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation
Cloning of the cro-box into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (all 7 of them) were transferred to a new plate containing ampicilin
- Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation
Ania
Tasks:
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