| Location | Strain # | Name | Chassis/Strain | Plasmid Backbone | Registry Part | Antibiotic Resistance | Description | Reference Date
|
| iGEM strain box 1
| 1
| rTT + PocI
| DH5α
| pMA (GeneArt)
| BBa_K093003
| Amp100
| Synthesized. Contains BioBrick prefix and suffix. Reverse orientation transcriptional terminator R0015 with λ Pr promotor.
| 2008:09:09
|
| iGEM strain box 1
| 2
| rPlacTT
| DH5α
| pMA (GeneArt)
| BBa_K093004
| Amp100
| Synthesized. Contains BioBrick prefix and suffix. Reverse BBa_R0011 and transcriptional terminator BBa_B0015.
| 2008:09:09
|
| iGEM strain box 1
| 3
| PmeI
| DH5α
| pMA (GeneArt)
| BBa_K093002
| Amp100
| Synthesized ORF. Contains BioBrick prefix and suffix.
| 2008:09:09
|
| iGEM strain box 1
| 4
| rfp'-14
| DH5α
| pSB1A2
| BBa_K093005
| Amp100
| RFP (BBa_E1010) with RBS (BBa_E0034) colony 14.
| 2008:09:09
|
| iGEM strain box 1
| 5
| rfp-2
| DH5α
| pSB1AK3
|
| Amp100, Kan25
| RFP (BBa_E1010) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) colony 2. Might need testing
| 2008:09:09
|
| iGEM strain box 1
| 6
| Pconst-rfp-1
| DH5α
| pSB1A2
| BBa_K093012
| Amp100
| RFP (BBa_E1010) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) under a constitutive promoter (BBa_J23118). Might need testing.
| 2008:09:09
|
| iGEM strain box 1
| 7
| pRecA-3
| DH5α
| psb2k3
| BBa_K093000
| Kan25
| Engineered RecA promoter with lexA consensus sequence.
| 2008:10:17
|
| iGEM strain box 1
| 8
| Pconst-rfp' 1
| DH5α
| psb1a2
|
| Amp100
| RFP (BBa_E1010) with RBS (BBa_E0034) under a constitutive promoter (BBa_J23118)
| 2008:10:17
|
| iGEM strain box 1
| 9
| c0051'
| DH5α
| psb1a2
| BBa_K093006
| Amp100
| BBa_C0051 Repressor for Lambda Pr promoter with RBS (BBa_E0034). Never got sequencing results of prefix. Rest was good
| 2008:10:17
|
| iGEM strain box 1
| 10
| PmeI - 14
| DH5α
| psb2k3
| BBa_K093002
| Kan25
| PmeI cloned into BioBrick vector
| 2008:10:17
|
| iGEM strain box 1
| 11
| PrecA RFP'
| DH5α
| pSB2K3
| BBa_K093009
| Kan25
| RFP (BBa_E1010) with RBS (BBa_E0034) under the control of BBa_K093000 promoter
| 2008:10:17
|
| iGEM strain box 1
| 12
| PmeI'
| DH5α
| pSB1A2
| BBa_K093013
| Amp100
| PmeI (BBa_K093002) with RBS (BBa_E0034)
| 2008:11:04
|
| iGEM strain box 1
| 13
| Pr RFP'
| DH5α
| psb1a2
| BBa_K093010
| Amp100
| RFP (BBa_E1010) with RBS (BBa_E0034) under Lamda Pr promoter (R0051)
| 2008:11:04
|
| iGEM strain box 1
| 14
| T7 gene 6
| DH5α
| pSB1A2
| BBa_K093001
| Amp100
| good one, correct stop codon taataa
| 2009:02:13
|
| iGEM strain box 1
| 15
| PkNG101
| DH5α λ pir
|
|
| Strep100
| Only replicates in λ pir lysogen (r6k origin of replication) http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pKNG101
| 2009:02:xx
|
| iGEM strain box 1
| 16
| H1-4 pJET
| DH5α
| pJET
|
| Amp100
| E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
| 2009:03:18
|
| iGEM strain box 1
| 17
| CI pJET
| DH5α
| pJET
|
| Amp100
| CI repressor for lamda Pr promoter. BBa_C0051 with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) in pJET (made by crossover PCR)
| 2009:03:xx
|
| iGEM strain box 1
| 18
| pFB9002
| DH5α lamda pir
| pfb9001
|
| Strep 100
| E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
| 2009:03:xx
|
| iGEM strain box 1
| 19
| pJET H2
| DH5α
| pJET
|
| Amp100
| E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
| 2009:04:09
|
| iGEM strain box 1
| 20
| Pfb9003
| DH5α lamda pir
| pfb9001
|
| Strep100
| E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
| 2009:04:23
|
| iGEM strain box 1
| 21
| pfb9004
| DH5α lamda pir
| pfb9001
|
| Strep100
| Contains two attP sites flanked by E. coli melA genomic DNA for homologous recombination (H1 and H2)
| 2009:04:23
|
| iGEM strain box 1
| 22
| pJET rfp-1
| DH5α
| pJET
|
| Amp100
| insert: Pconst (BBa_J23118) rfp (BBa_E1010) EcoRV fragment for propagating pJET
| 2009:04:24
|
| iGEM strain box 1
| 79
| pJET rfp-2
| DH5α
| pJET
|
| Amp100
| insert: Pconst rfp EcoRV fragment
| 2009:04:24
|
| iGEM strain box 1
| 81
| pJET rfp-3
| DH5α
| pJET
|
| Amp100
| insert: Pconst rfp EcoRV fragment
| 2009:04:24
|
| iGEM strain box 1
| 23
| RBS
| DH5α
| pSB1A2?
| B0034
| Amp100
| Ribosome binding site (Elowitz)
|
|
| iGEM strain box 1
| 24
| Pconst55%
| DH5α
| BBa_J61002
| BBa_J23118
| Amp100
| Constitutive promoter. Expression level is 55% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
|
|
| iGEM strain box 1
| 25
| TT
| DH5α
| pSB1AK3
| BBa_B0015
| Amp100, Kan25
| Default double transcriptional terminator
|
|
| iGEM strain box 1
| 26
| rfp
| DH5α
| pSB2K3
| E1010
| Kan25
| Red fluorescent protein (shows up pink even without appropriate wavelength of light and filter)
|
|
| iGEM strain box 1
| 27
| Pseudomonas mendocina
| kr 1
|
|
| ?
| Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1
| 2008:08:xx
|
| iGEM strain box 1
| 28
| Plac
| DH5α
| pSB1A2
| BBa_R0010
| Amp100
| Wildtype LacI repressable promoter
|
|
| iGEM strain box 1
| 29
| PtetR
| DH5α
| pSB1A2
| BBa_R0040
| Amp100
| Promoter that is repressed by tetR and derepressed by tetracyclin.
|
|
| iGEM strain box 1
| 30
| CI
| DH5α
| pSB1A2
| BBa_C0051
| Amp100
| CI repressor for λ Pr promoter
|
|
| iGEM strain box 1
| 31
| rTT
| DH5α
| pSB1A2
| B0025
| Amp100
| Reverse transcriptional terminator (BBa_B0015)
| 2008:xx:xx
|
| iGEM strain box 1
| 32
| Taq
| DH5α
|
|
| Amp100
| Taq strain from Moffatt lab for purifying taq
| 2008:08:xx
|
| iGEM strain box 1
| 33
| Pconst+
| DH5α
| pSB1A2
| BBa_J23119
| Amp100
| Wildtype promoter─strongest of this promoter family (BBa_J23100-BBa_J23119)
|
|
| iGEM strain box 1
| 34
| LacI
| DH5α
| pSB1A2
| BBa_C0012
| Amp100
| LacI repressor for Plac Promoter (BBa_R0010) with LVA degredation tag
|
|
| iGEM strain box 1
| 35
| Pconst4%
| DH5α
| BBa_J61002
| BBa_J23109
| Amp100
| Constitutive promoter. Expression level is 4% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
|
|
| iGEM strain box 1
| 36
| TTbi
| DH5α
| pSB1AK3
| BBa_B0014
| Amp100,Kan25
| Double terminator─not as strong as BBa_B0015 but more even efficiency for forward and reverse use.
|
|
| iGEM strain box 1
| 37
| Pconst100%
| DH5α
| BBa_J61002
| BBa_J23100
| Amp100
| Constitutive promoter. Expression level is 100%─the highest expression level for this Pconst family (i.e., wildtype promoter)
|
|
| iGEM strain box 1
| 38
| PlacCI
| DH5α
| pSB2K3
|
| Kan25
| cI repressor (BBa_C0051) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) under the promoter Plac (BBa_R0011).
| 2009:04:xx
|
| iGEM strain box 1
| 39
| pTC300
| DH5α
| pUC19
|
| Cm10, Kan25
| Strain containing Kanamycin resistance gene, and SacB negative selection gene flanked together by BamHI sites
| 2009:05:30
|
| iGEM strain box 1
| 40
| DB 3.1
| DB 3.1
|
|
|
| CCDB resistant strain, contains mutation in GyrA
| 2009:05:30
|
| iGEM strain box 1
| 41
| DH5α
| DH5 alpha
|
|
|
| For making competent cells. Default strain for
| 2007:xx:xx
|
| iGEM strain box 1
| 42
| pFB9006
| DH5α λ pir
| pFB9001
|
| Strep100/Kan20
| pFB9005+KanSac. pFB9001 containing H2 homologous region to E. coli melA gene. Does not contain H1 region.
| 2009:06:xx
|
| iGEM strain box 1
| 43
| pSB1A3
| DH5α
| pSB1A3
|
| Amp 100
| BioBrick vector from 2009 registry.
| 2009:06:16
|
| iGEM strain box 1
| 44
| luxI
| DH5α
| pSB1AK3
|
| Amp100, Kan 25??
| luxI (BBa_C0061) +RBS (BBa_E0034) and transcriptional terminator (BBa_B0015)
| 2007:xx:xx
|
| iGEM strain box 1
| 45
| PlaclasR
| DH5α
| pSB1A2
|
| Amp100
| lasR (C0079) with RBS (BBa_E0034) and TT (BBa_B0015) under the control of a lac promoter (unsure which promoter - would need testing/sequencing)
| 2007:xx:xx
|
| iGEM strain box 1
| 46
| Plux rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP (BBa_E1010) with RBS (BBa_E0034) under Plux promoter
| 2007:xx:xx
|
| iGEM strain box 1
| 47
| Plas rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP (BBa_E1010) with RBS (BBa_B0034) under Plas promoter (BBa_R0079)
| 2007:xx:xx
|
| iGEM strain box 1
| 48
| Ptet rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP (BBa_E1010) with RBS (BBa_B0034) under Ptet promoter (BBa_R0040)
| 2007:xx:xx
|
| iGEM strain box 1
| 49
| PompR rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP (BBa_E1010) with RBS (BBa_B0034) under PompR promoter
| 2007:xx:xx
|
| iGEM strain box 1
| 50
| tetR
| DH5α
| pSB2K3
|
| Kan25
| tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
| 2007:xx:xx
|
| iGEM strain box 1
| 51
| ???
| DH5α
| pSB2K3
|
| Kan25
|
|
|
| iGEM strain box 1
| 52
| lasI
| DH5α
| pSB2K3
|
| Kan25
| lasI (BBa_C0078) with RBS (BBa_E0034) and TT (BBa_B0015)
| 2007:xx:xx
|
| iGEM strain box 1
| 53
| tetR
| DH5α
| pSB2K3
|
| Kan25
| tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
| 2007:xx:xx
|
| iGEM strain box 1
| 54
| tetR'
| DH5α
| pSB1A2
|
| Amp100
| tetR repressor (BBa_C0040) with RBS (BBa_B0034)
| 2007:xx:xx
|
| iGEM strain box 1
| 55
| lasR
| DH5α
| pSB1A2
|
| Amp100
| lasR (BBa_C0079) with RBS (BBa_B0034) and TT (BBa_B0015)
| 2007:xx:xx
|
| iGEM strain box 1
| 56
| Plac IA7K
| DH5α
| pSB1A2
|
| Amp100
| ????
|
|
| iGEM strain box 1
| 57
| pSB1A2 attB TG
| DH5α
| pSB1A2
|
| Amp100
| Was previously Pr RFP', RFP was cut out and attB TG was inserted. Flanked by EcoRI and PstI sites.
| 2009:07:06
|
| iGEM strain box 1
| 58
| pSB1A2 attB TT
| DH5α
| pSB1A2
|
| Amp100
| Same as Strain #57, except the inserted fragment was attB TT instead. This is the wild-type attB.
| 2009:07:06
|
| iGEM strain box 1
| 59
| pSB1A2 attB CT
| DH5α
| pSB1A2
|
| Amp100
| Same as Strain #57, except the inserted fragment was attB CT instead.
| 2009:07:06
|
| iGEM strain box 1
| 60
| rPlacTT + PlacCI
| DH5α
| pSB2K3
|
|
|
| 2009:06:15
|
| iGEM strain box 1
| 61
| 2008 Construct w/o Promoter
| DH5α
| pSB1A2
|
| Amp100
| RBS + Pmel + rPlacTT + PlacCI. 2008 project.
| 2009:07:10
|
| iGEM strain box 1
| 62
| 2008 Constant
| DH5α
|
|
| Amp100
|
| 2009:07:15
|
| iGEM strain box 1
| 63
| pSB3C5
| DB3.1
| pSB3C5
|
| Cm25
| Low copy # plasmid, no promoter, no RBS, "CCDD" (BBa 15001/15002)?
|
|
| iGEM strain box 1
| 64
| BBa
|
| pSB3C5
|
|
|
|
|
| iGEM strain box 1
| 65
| pKNG101
| DH5α
| pKNG101
|
| Strep100
| Plasmid backbone for the pFB9xxx series.
| 2009:08:07
|
| iGEM strain box 1
| 66
| DH5α λ pir
| DH5 alpha lambda pir
| None
|
| None
| DH5α λ pir stock for making competent cells.
| 2009:07:24
|
| iGEM strain box 1
| 67
| pFB9007
| DH5α λ pir
| pFB9001
|
| Strep100
| Contains H2 region and an attP site. Same as pFB9004 except w/o the truncated SacB gene on the plasmid backbone.
| 2009:08:05
|
| iGEM strain box 1
| 68
| pFB9008
| DH5α λ pir
| pFB9007
|
| Strep100
| Same as pFB9007, but it has RFP cloned in upstream of the attP site and the H2 region.
| 2009:08:05
|
| iGEM strain box 1
| 69
| pFB9009 #2
| DH5α λ pir
| pFB9008+H1
|
| Strep100/Kan20
| Same as pFB9008+H2, but it has Kanr and sacB genes cloned in between the H1&H2 regions, directly downstream adjacent to the RFP.
| 2009:08:28
|
| iGEM strain box 1
| 70
|
|
|
|
|
|
|
|
| iGEM strain box 1
| 71
| pSB4A5
| DH5α
| pSB4A5
|
| Amp100
| Low copy number, no promoter, no RBS.
| 2009:08:28
|
| iGEM strain box 1
| 72
| pSB4C5
| DB3.1
| pSB4C5
|
| Cm25
| Low copy number, no promoter, no RBS.
| 2009:08:28
|
| iGEM strain box 1
| 73
| OriT-L #13
| DH5α
| pSB1A2
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 74
| OriT-L #18
| DH5α
| pSB1A2
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 75
| BBa_J23116 Pconst
| DH5α
| BBa_J61002
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 76
| BBa_J23118 Pconst
| DH5α
| BBa_J61002
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 77
| pFB9008+H1 #13
| DH5α λ pir
| pFB9007
|
| Strep100/Kan20
| Same as pFB9008, except that H1 region and its attP site are cloned in upstream of the RFP gene to flank the RFP. Low copy number.
| 2009:08:28
|
| iGEM strain box 1
| 78
| pSB4C5-1
| DB3.1
| pSB4C5
|
| Cm25
|
| 2009:08:20
|
| iGEM strain box 1
| 79
| pSB4C5-2
| DB3.1
| pSB4C5
|
| Cm25
|
| 2009:08:20
|
"
