Team:Wisconsin-Madison/Cloning Protocols


Overview of Cloning Protocol

Insert and Vector

  • Solid Culture
  • Liquid Culture
  • Plasmid Isolation
  • Restriction Enzyme Mapping for Verification

Insert Only

  • PCR to add BioBrick Restriction Cut Sites (Primer Design)

Insert and Vector

  • PCR Plasmid Cleanup
  • Concentration Verification
  • Digestion
  • Digestion Clean Up
  • Concentration Verification
  • Ligation
  • Transformation
  • Colony PCR Screening

** Procedures for these can be found in Protocol Section! **
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