Template:Team:KULeuven/21 September 2009/BlueLightReceptor

From 2009.igem.org

  1. a new restriction digest on logA (1&2) was done.
  2. the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. the signals were good.
  3. a glycerol stock of ligY was made and some samples for sequencing were prepared.
  4. a motherplate of LogA en LikA were made and liquid cultures to make a glycerol stock.
  5. liquid cultures were also made for empty top10 cells, standard promotor and LikA (blp and GFP in pSB3K3).