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Template:Team:KULeuven/24 August 2009/BlueLightReceptor
From 2009.igem.org
- Plates with LigA were put under blue light. The LEDs were put on their max capacity.
- Restriction digest with
- tubes (1,3,5,7,9) of LigA (BLP + BBa_E0240) cut with EcoRI en PstI
- promotor BBa_J23101 cut with EcoRI en SpeI (4x)
- Gel electrophoresis with the RD of LigA and BBa_J23101 followed by a gel extraction:
- Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
- Note: the samples of promotor BBa_J23101 were barely visible. Only 2 of the 4 samples were recovered by extraction.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA 1 | 7,9 | 2,16 | |
| LigA 3 | 10,8 | 1,88 | |
| LigA 7 | 4,8 | 2,49 | |
| LigA 9 | 8,0 | 3,02 | |
| BBa_J23101 (A) | 20,8 | 1,70 | |
| BBa_J23101 (B) | 12,8 | 2,57 |
4. Enting of liquid cultures with kanamycin and pSB3K3
