Template:Team:KULeuven/27 August 2009/BlueLightReceptor

From 2009.igem.org

  1. The electroporations of 26/08 were checked.
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had some colonies. they were ented in liquid culture.
    • LigC (Content-type: text/html

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) did not grow. we figured that an insert of 35bp was too short to ligate so we decided to use Content-type: text/html

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as insert and Content-type: text/html

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as vector . The following restriction digest was started:
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was cut with SpeI and PstI
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was cut with XbaI and PstI  
  1. The miniprep that was made to sequence the LigA construct on 26/08 was used again to perform a restriction digest (EcoRI and PstI). This was put on gel to check whether there actually was LigA-insert in the vector and whether the insert had the right length. The gel showed a signal at 1000 bp and at 2000 bp which coincides with the insert (BLp + GFP) and the vector.
  2. A new setup to light the E.coli was engineered.
    • Fresh cultures were made from the old ones (LB plate ligA 14/08 and the two liquid cultures from 26/08 were used as templates.)
    • They were put in the 16°C room for about an hour
    • A blue light (40W) was put on them for about an hour
    • They were put in the 37°C incubator overnight