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Template:Team:KULeuven/3 August 2009/VanillinProduction
From 2009.igem.org
We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
- sam5 + RBS in pSB1A2 (with PstI site removed), BBa_K238007
- sam8 + RBS in pSB1A2, BBa_I742106
- sam8 in pSB1A2 coding sequence, BBa_I742142
- COMT in pSB1A2 coding sequence, BBa_I742107
The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.
