Template:Team:KULeuven/4 September 2009/BlueLightReceptor

From 2009.igem.org

  1. Gel extraction performed on the signals from 3/09
    • Nanodrop: 14,7 ng/µl of BBa_J61002 with a 260/280 of approx 2,12
  2. Ligation with BBa_J61002 and BBa_K238013 (ligX 1)
  3. A new restriction digest on BBa_J61002 and BBa_K238013 with EcoRI and SpeI was performed.
  4. Gel extraction of these new digests. BBa_J61002 was nanodropped with a concentration of 28 ng/µl. this was ligated with BBa_K238013 (ligX 2)
  5. Electroporation of pSB3K3
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