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Template:Team:KULeuven/5 August 2009/VanillinProduction
From 2009.igem.org
Miniprep of pairs:
- RBS + sam5 BBa_K238007 conc: 101,3 ng/ul
- RBS + sam8 BBa_I742106 conc: 95.7 ng/ul
- sam8 BBa_I742142 conc: 81,8 ng/ul
- COMT BBa_I742107, conc: 122,9 ng/ul
Restriction digestion
A1: sam8, EcoRI en SpeI
A2: sam5, EcoRI en XbaI
B1: RBS, EcoRI en SpeI
B2: COMT, EcoRI en XbaI
C1: fcs, EcoRI en SpeI
C2: ech, EcoRI en XbaI
The mixture was incubated in 37°C for 1h30...
...followed by gel electrophoresis of the digested parts
