The Encapsulator Model

From 2009.igem.org

Reactions

Reaction Rate Constant
Constitutive Expression
--> lacl4 1E-10
--> tetR2 1E-10
Multimerization
2 lacI --> lacI2 1E+09
lacI2 --> 2 lacI 10
2 lacI2 --> lacI4 1E+09
lacI4 --> 2 lacI2 10
2 tetR --> tetR2 1E+09
tetR2 --> 2 tetR 10
Transcription
RNAp + BBa_R0010 + LacI_binding_site --> RNAp:BBa_R0010:LacI_binding_site 10000000
RNAp:BBa_R0010:LacI_binding_site --> RNAp + BBa_R0010 + LacI_binding_site 0.057
RNAp:BBa_R0010:LacI_binding_site --> RNAp:BBa_R0010:LacI_binding_site* 0.1
RNAp:BBa_R0010:LacI_binding_site* --> RNAp:DNA_eCFPt + BBa_R0010 + LacI_binding_site 30
RNAp:DNA_eCFPt --> RNAp + mRNA_eCFPt 0.035
RNAp + BBa_R0040 + TetR_1 + TetR_2 --> RNAp:BBa_R0040:TetR_1:TetR_2 10000000
RNAp:BBa_R0040:TetR_1:TetR_2 --> RNAp + BBa_R0040 + TetR_1 + TetR_2 0.057
RNAp:BBa_R0040:TetR_1:TetR_2 --> RNAp:BBa_R0040:TetR_1:TetR_2* 0.1
RNAp:BBa_R0040:TetR_1:TetR_2* --> RNAp:DNA_Enc + BBa_R0040 + TetR_1 + TetR_2 30
RNAp:DNA_Enc --> RNAp + mRNA_Enc 0.0375
Translation
rib + mRNA_eCFPt --> rib:mRNA_eCFPt 100000
rib:mRNA_eCFPt --> rib:mRNA_eCFPt_1 + mRNA_eCFPt 33
rib:mRNA_eCFPt_1 --> rib + eCFPt 0.1154
rib + mRNA_Enc --> rib:mRNA_Enc 100000
rib:mRNA_Enc --> rib:mRNA_Enc_1 + mRNA_Enc 33
rib:mRNA_Enc_1 --> rib + Enc 0.1234
Protein-DNA
lacI4 + nsDNA --> lacI4:nsDNA 1000
lacI4:nsDNA --> lacI4 + nsDNA 1.6225
lacI4 + LacI_binding_site --> lacI4:LacI_binding_site 1E+09
lacI4:LacI_binding_site --> lacI4 + LacI_binding_site 0.005
tetR2 + nsDNA --> tetR2:nsDNA 1000
tetR2:nsDNA --> tetR2 + nsDNA 1.6225
tetR2 + TetR_1 --> tetR2:TetR_1 1E+09
tetR2:TetR_1 --> tetR2 + TetR_1 0.005
tetR2 + TetR_2 --> tetR2:TetR_2 1E+09
tetR2:TetR_2 --> tetR2 + TetR_2 0.005
Protein - Effector - DNA
lacI4 + IPTG --> lacI4:IPTG 50000000
lacI4:IPTG --> lacI4 + IPTG 0.1
lacI4:IPTG + LacI_binding_site --> lacI4:IPTG:LacI_binding_site 1E+09
lacI4:IPTG:LacI_binding_site --> lacI4:IPTG + LacI_binding_site 0.7
lacI4:LacI_binding_site + IPTG --> lacI4:IPTG:LacI_binding_site 1000000
lacI4:IPTG:LacI_binding_site --> lacI4:LacI_binding_site + IPTG 0.4
lacI4:IPTG + nsDNA --> lacI4:IPTG:nsDNA 1000
lacI4:IPTG:nsDNA --> lacI4:IPTG + nsDNA 1.6225
lacI4:nsDNA + IPTG --> lacI4:IPTG:nsDNA 1000
lacI4:IPTG:nsDNA --> lacI4:nsDNA + IPTG 1.6225
tetR2 + aTc --> tetR2:aTc 50000000
tetR2:aTc --> tetR2 + aTc 0.1
tetR2:aTc + TetR_1 --> tetR2:aTc:TetR_1 1E+09
tetR2:aTc:TetR_1 --> tetR2:aTc + TetR_1 0.7
tetR2:TetR_1 + aTc --> tetR2:aTc:TetR_1 1000000
tetR2:aTc:TetR_1 --> tetR2:TetR_1 + aTc 0.4
tetR2:aTc + TetR_2 --> tetR2:aTc:TetR_2 1E+09
tetR2:aTc:TetR_2 --> tetR2:aTc + TetR_2 0.7
tetR2:TetR_2 + aTc --> tetR2:aTc:TetR_2 1000000
tetR2:aTc:TetR_2 --> tetR2:TetR_2 + aTc 0.4
tetR2:aTc + nsDNA --> tetR2:aTc:nsDNA 1000
tetR2:aTc:nsDNA --> tetR2:aTc + nsDNA 1.6225
tetR2:nsDNA + aTc --> tetR2:aTc:nsDNA 1000
tetR2:aTc:nsDNA --> tetR2:nsDNA + aTc 1.6225
Degradation
lacI4 --> 0.000289
tetR2 --> 0.000289
mRNA_eCFPt --> 0.0015
mRNA_Enc --> 0.0015
eCFPt --> 0.000289
Enc --> 0.000289
Dilution
lacI4:nsDNA --> nsDNA 0.000193
tetR2:nsDNA --> nsDNA 0.000193
lacI4:IPTG --> IPTG 0.000289
tetR2:aTc --> aTc 0.000289
lacI4:IPTG:nsDNA --> IPTG + nsDNA 0.000193
tetR2:aTc:nsDNA --> aTc + nsDNA 0.000193