Mercury Vacuum Project
01/09/09 - Agarose Gel and Nanodrop
- Miniprepped BBa_J63010 plasmids ran on 1% Agarose gel (TAE buffer, pH8.5) at 200V, 70mA for 1hour. Post-stain: Ethidium Bromide. Click HERE for a picture of the gel. Lanes1,8 - empty, Lane2 - ladder, Lanes3-7 - Miniprepped DNA (colonies A-E)
- Miniprepped BBa_J63010 plasmids (tubes A to E) analysed on nanodrop. (Data printout in IGEM_Mercury notebook)
- New space created for BBa_J63010 tubes A to E: in "Fiona" freezer, UQ-Australia IGEM Team's Green box.
- In the afternoon, digests of samples BBa_J63010 A-E (Standard 23) were run with EcoRI, AgeI, SpeI and PstI enzymes. Unfortunately NgoMIV enzymes were not in stock in the freezer so this digest could not be performed.
- The Standard 21 plasmid BBa_I716101 was again digested with PstI. All samples were left in the 37 degrees water bath overnight.
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Bioprecipitation Project
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