Uppsala-Sweden/17 July 2009

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Ethanol survival experiment

10:00 OD measurement. One E-flask 0 vol% was tipped over during loading of measurement plate. Readings performed on one time diluted culture. The 0 and 1 vol% concentrations shows growth since last reading, the others have unchanged OD. In the 2 and 4 vol% cultures the cells have formed clumps, survival mechanism. The 8 vol% is visibly unchanged since yesterday.

Continued problems with fogging on plate, don't want to wipe fog as large amounts of accumulated ethanol would be lost. Measurement taken without plate lid --> not sterile. In wells across plate with low correlation to ethanol concentration cells have formed clupms, ethanol contamination through shared atmosphere? This further disrupts the readings as the cells are concentrated to the middle of the well giving abnormally high readings.

15:30 OD measurement. Same trend as earlier this day. Should have clear results by Monday morning.

/Anders

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