Uppsala-Sweden/17 September 2009

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Colony PCR of yesterdays transformations

The colony PCR was this time performed utilizing dream taq.

Weseem to have good transformations of R-Kivd (2, 5), KRYT (4, 5, 6). Quite a meager turnout of that many transformations, something still screws up the process.

Pictures:

20090917 colonypc.jpg

inoculate RKivd, 2 5, KRYT 4, 5, 6 only. --Anders 15:11, 21 September 2009 (UTC)

Evaluation of EcorI, SpeI and Adh2Z

Due to poor transformation efficiency we decided to evaluate the restriction enzymes by single and double digestion of RPRAT

The result:

Es eva 090917 t.jpg

The triple bands from the RPRA EcorI digestion can be explained by additional restriction by that enzyme forming Single and double restriction products. ~6kb plasmid + RPRAT, ~3.3 kb plasmid + RPRAT fragment, ~2.7 kb RPRAT fragment.

We conclude fault in EcorI or RPRAT.

--Anders 20:56, 19 October 2009 (UTC)

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