Uppsala-Sweden/30 September 2009

From 2009.igem.org




Horrible Transformation results

All the transformations forma yesterday failed, the purified digestions were run on a gel to figure out if the problem were related to us loosing all the DNA in the purification step.

The result turned out to be as we suspected, basically all the DNA was gone.

090929 cut eval 2.png


Evaluation of Nucleospin PCR Clean-Up kit

DUe to the results we decided to evaluate the Nuclespin kit, we used different columns, amount of water, wash buffer and dilutions of NT buffer. Nucleospin eval table.png


The result was that there is no difference in we use our own wash buffer or other binding columns. But if the NT buffer is diluted the recovery is way much lower than what is said in the manual for DNA fragments of our size.




Bioneer Biolegio Clontech Uppsala Genome Center

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