Uppsala-Sweden/7 August 2009

From 2009.igem.org




Overview sheet

Media:Transformation Workorder.pdf

Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3

Ligation with the pSB1A3 vector using the Quick Ligation Kit from NEB. Calculated amounts can be found here Media:Ligation Calc 07082009.xls

Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells

Transformation was performed as written in the protocol form the Ligation kit from NEB

--Karl.brune 08:56, 10 August 2009 (UTC)