Uppsala-Sweden/7 September 2009

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Contents

Plasmid Preps

Plasmid Prep with Sigma Gene Elute was performed with the following samples:

Subunit E2

  1. E21-1
  2. E22-3
  3. E23-3
  4. E24-1

Subunit E1 alpha

  1. E1A1-1
  2. E1A1-2
  3. E1A2-2
  4. E1A2-3
  5. E1A3-1
  6. E1A4-2

Subunit E1 beta

  1. E1B1-3
  2. E1B2-3
  3. E1B3-1
  4. E1B4-2

B0015, B0034-pirA, R0011-B0034, B0034-Yadh2-B0015

  1. T-1 - inoculation failed, new inoculation was performed
  2. RP-2
  3. RR-4
  4. RR-5
  5. RYT-1
  6. RYT-2

Furthermore these were prepped (Lux System etc.)

A340620, C0621, J237034

  1. A340620
  2. C0621
  3. J237034

and these here:

  1. R0062 (A)
  2. RARP 4, 5 ,7 (CH)

Plasmid Prep with Sigma Gene Elute was performed with the following samples:

RBS-pdc-RBS-ADH2(z) Three plasmid preps were performed for the large construct RBA-pdc-RBS-ADH2(z). With the final concenttations (100-td)

Media:Plasmidprep_090907.TXT

The result was evaluated by cutting with EcoR1 and PstI and running on a gel.

RPRAplasmidevaluationcutEP.jpg

--Flormane 06:27, 8 September 2009 (UTC)


--Karl.brune 14:12, 7 September 2009 (UTC)

Report for the above preps: Media:Plasmidprep_090907.txt --Anders 17:49, 7 September 2009 (UTC)

2nd Plasmid Prep

Prep of the B0015 (AC3) culture from this morning

--Karl.brune 21:50, 7 September 2009 (UTC)

Pouring Plates

I poured plates ( Kanamycin and Chloramphenicol ), stored at iGEM fridge

--Karl.brune 21:50, 7 September 2009 (UTC)

Inoculation for PP

  1. pSB1A3
  2. pSB1AC3 (2)
  3. pSB1AK3 (2)
  4. B0034 (A) (2)
  5. I13504 (A) (2)

Overnight at 37C, 250RPM in 6ml LB with specific antitbiotic

--Karl.brune 21:50, 7 September 2009 (UTC)

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