User:DavidC/21 August 2009

From 2009.igem.org

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Contents

Friday the 21th

PCR

PCR of adenovirus 5 penton base from plasmid coding for the virus.

94°C, 2 minute.
(94°C, 1 minute.
55°C, 1 minutes.
72°C, 3 minutes.) X 5
72°C, 5 minutes.
(94°C, 1 minute.
66°C, 1 minutes.
72°C, 3 minutes.) X 30

DNA electrophoresis

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
sample: adenovirus penton base (ADV5pb)

F2108.png

No amplification of the adenovirus 5 penton base, we have mismatch on the second trial.

Lambda phage extraction

Lambda phage extraction. 2 dishes, at 12 and 24 hours of incubation, dilution = 1/10 and 1/10 000.

Growth of lambda phages for extraction. 5 dishes with E.coli infected by Lambda phages, dilution = 1/10.