User:DavidC/27 August 2009

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Contents

Thursday the 27th

DNA extraction

Samples : C0012 = 195 ng/µL; R0040 = 130ng/µL; B0030 = 270ng/µL; P1002 (1)= 80ng/µL; P1002(2)= 200ng/µL.

First restriction digest:

Brick : BBa_R0040

DNA (1µg) = 7,69µL
Buffer Takara H 10x = 2µL
H20 = 8,31µL
Spé 1 (Takara) = 1µL
Eco R1 (Takara) = 1µL
1h at 37°C.

Brick : BBa_B0030

DNA (1µg) = 3,64 µL
Buffer Takara H 10x = 2µL
H20 = 12,36µL
Xba 1 (Takara) = 1µL
Eco R1 (Takara) = 1µL
1h at 37°C.

DNA electrophoresis

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.

No results, we do not have any plasmid restriction digest.