From 2009.igem.org
Thursday 11 June 2009
Results
- Note these were plated Wednesday evening with two groups splitting the work.
- Group 1: Craig, Clay, Afton plates
- Observations on the Controls
| Control | Plate Type | Observation |
|
| NEB10β (+)
| LB | lawn |
|
| NEB10β (-)
| Cm | no growth |
|
| NEB10β (-)
| Amp | no growth |
|
| NEB10β (-)
| Kan | 3 colonies |
|
- Growth on Kan could have resulted from a non-homogeneous medium with very small sections of the plate being able to sustain life. Or there was degradation of the Kan-selective medium.
- Observations on the Colonies
| Part No. | Plate Type | Colonies | Other Observations |
|
| pSB 3K3
| Kan | 10s of colonies | n/a |
|
| E0240
| Amp | 100s of colonies | no color |
|
| J23101
| Amp | 1000s of colonies | Pink in color. |
|
| J23113
| Amp | 100s of colonies | n/a |
|
| J23116
| Amp | 100s of colonies | n/a |
|
| J23102
| Amp | 10s of colonies | Pink in color. |
|
| R0040
| Amp | 10s of colonies | n/a |
|
- Group 2: Adam, Kevin and Maria Plates
- Observations on the Colonies
| Part No. | Plate Type | Colonies | Other Observations |
|
| R0011
| Amp | 10s of colonies | n/a |
|
| J23100
| Amp | 10s of colonies | Slightly pink |
|
| J23103
| Amp | low 100s of colonies | Pink in color |
|
| J23104
| Amp | 100s of colonies | n/a |
|
| J23105
| Amp | 100s of colonies | n/a |
|
| J23107
| Amp | 1000s of colonies | n/a |
|
| J23110
| Amp | 1000s of colonies | n/a |
|
- Problems:
- Part J23106 was taken from well 18D, when it actually is located in well 18O.
- Part Q04511 was actually taken from well 18D
- Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.
MandM 22:52, 11 June 2009 (UTC)
Tasks
- Literature Discussion
- Discussion of iGEM ideas
Wetlab
- Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL)
MandM 22:55, 11 June 2009 (UTC)
|
"
