Virginia Commonwealth/13 July 2009

From 2009.igem.org

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Contents

Monday 13 July 2009

Results

Maria and Afton

  • Digestion was successful on parts J23106 and J06702
  • Gel Electrophoresis Results
from left to right: 100bp Ladder,J23106,J06702,J06702

Trentay 18:30, 13 July 2009 (UTC)

Craig and Clay

  • The parts that were plated on Friday were unsuccessful. Electrotransformation will be performed again.

Tasks

Maria and Afton

  • Make daily planner for experimentation
  • Perform Electrophoresis test on Digested parts J23106 and J06702
  • Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
  • Transform ligated DNA into e. coli NEB 10 beta

Trentay 18:34, 13 July 2009 (UTC)

Kevin and Adam

  • Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock.


Craig and Clay

  • A second attempt at electrotransformation of parts BBa_I742111, BBa_K118024, and BBa_K118025 will be performed today. These electrotransformed parts will be plated on ampicillin plates.
  • If the above is unsuccessful, the parts will be re-plated on the ampicillin plates and on non-selective plates.

Wetlab

Maria and Afton

  • Gel Electrophoresis
  • Digestion of J23100
  • Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
  • Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone)
  • Plated, Controls and NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
Name Plate Growth Observation
Control (+) NEB10β LB n/a
Control (+) NEB10β shocked LB n/a
Control (-) NEB10β Cm n/a
J23106/J06702/pSB4C5 ligation Cm n/a

Trentay 20:26, 13 July 2009 (UTC)

Kevin and Adam

  • grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning.

Bussingkm 22:59, 13 July 2009 (UTC)

Craig and Clay

  • The three parts related to limonene synthesis were electrotransformed again and plated on ampicillin plates.
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