Virginia Commonwealth/13 June 2009

From 2009.igem.org

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Contents

Saturday 13 June 2009

Results

  • Overnight cultivations were successful

Tasks

  • Mini-prep backbone pSB3k3 and reporter E0240
  • Freeze cells from overnight culture for storage

Wetlab

  • Mini-Prep results
Part No. A260 A260/A280 A280 Volume (uL) Conc. (ug/mL) Amt. (ug)
pSB3K3 (1) 0.016 1.231 0.013 41.0 24.0 0.984
pSB3K3 (2) 0.020 1.111 0.018 41.2 30.0 1.236
pSB3K3 (3) 0.021 1.167 0.018 41.6 31.5 1.310
pSB3K3 (4) 0.030 1.154 0.026 43.0 45.0 1.935
E0240 (1) 0.020 1.217 0.023 40.0 30.0 1.200
E0240 (2) 0.025 1.190 0.021 42.0 37.5 1.575
E0240 (3) 0.030 1.200 0.025 41.5 45.0 1.868
E0240 (4) 0.033 1.269 0.026 39.0 49.5 1.931


  • Samples were diluted 30x for spectrophotometry. A260=50 (ug/mL)
  • Cell stock was frozen and stored in box C-15.
  • DNA was stored in the lab freezer.

-Bussingkm 15:52, 13 June 2009 (UTC)

Retrieved from "http://2009.igem.org/Virginia_Commonwealth/13_June_2009"