From 2009.igem.org
Friday 17 July 2009
Results
- Results of overnight culture was successful
- NEB 10 beta electrocompetency was successful
- NEB on LB had lawn growth
- NEB on LB after being shocked had lawn growth
- Electrophoresis test on J06702 and pSB4C5
- A DNA band was visible for both
Trentay 16:54, 17 July 2009 (UTC)
Craig and Clay
- There is still no growth on the kanamycin and chloramphenicol plates.
Tasks
Maria and Afton
- Religate promoters that did not grow in the first transformation (backbone pSB4C5)
- J23101 w/ E0240
- J23105 w/ E0240
- Run an electrophoresis test on J06702 and pSB4C5
Trentay 15:53, 17 July 2009 (UTC)
Wetlab
Maria and Afton
- Cryogenic stock of the following BioBricks was made with backbone pSB4C5 (2 vials each)
Promoter | RET
|
J23101 | J06702
|
J23102 | E0240
|
J23102 | J06702
|
J23103 | E0240
|
J23103 | J06702
|
J23104 | E0240
|
J23104 | J06702
|
J23105 | J06702
|
J23107 | E0240
|
J23107 | J06702
|
Trentay 15:53, 17 July 2009 (UTC)
|