Virginia Commonwealth/17 July 2009

From 2009.igem.org

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Contents

Friday 17 July 2009

Results

  • Results of overnight culture was successful
  • NEB 10 beta electrocompetency was successful
    • NEB on LB had lawn growth
    • NEB on LB after being shocked had lawn growth
  • Electrophoresis test on J06702 and pSB4C5
    • A DNA band was visible for both

Trentay 16:54, 17 July 2009 (UTC)

Craig and Clay

  • There is still no growth on the kanamycin and chloramphenicol plates.

Tasks

Maria and Afton

  • Religate promoters that did not grow in the first transformation (backbone pSB4C5)
    • J23101 w/ E0240
    • J23105 w/ E0240
  • Run an electrophoresis test on J06702 and pSB4C5

Trentay 15:53, 17 July 2009 (UTC)


Wetlab

Maria and Afton

  • Cryogenic stock of the following BioBricks was made with backbone pSB4C5 (2 vials each)
Promoter RET
J23101 J06702
J23102 E0240
J23102 J06702
J23103 E0240
J23103 J06702
J23104 E0240
J23104 J06702
J23105 J06702
J23107 E0240
J23107 J06702
  • Electrophoresis test

Trentay 15:53, 17 July 2009 (UTC)