From 2009.igem.org
Tuesday 18 August 2009
Results
Maria and Afton
- Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression
- Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink)
- Looking over the past results of transformations on June 11:
- Only J23100-104 expressed florescence on the plates
- The overnight cultures say, "all cells grew well"
- It is not 100% clear whether ALL of the cells with promoter parts ever expressed RFP
- The amount of time it takes for the cells to fluoresce will be monitored from now on
- Electrocompetency was completed
- 80 vials of 250µL Electrocompetent NEB10β was made
- They will be tested today to ensure the procedure was successful
Trentay 14:11, 18 August 2009 (UTC)
Tasks
Kevin
Maria and Afton
- Do Spectrophotometry of E0240 and pSB1C3
- Digest E0240 and pSB1C3 w/p1010
- Run a gel on E0240 and pSB1C3 w/p1010
- Ligate E0240 w/ pSB1C3 with J23100-104
- Digests from last week will be used for ligation
- However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations
- Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040
- Transform newly made electrocompetent cells to make sure they are working
Trentay 14:20, 18 August 2009 (UTC)
Wetlab
Maria and Afton
- Transformations were done
Trentay 02:25, 19 August 2009 (UTC)
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