Virginia Commonwealth/21 July 2009

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Contents

Tuesday 21 July 2009

Results

  • Overnight cultures were successful
  • Plated bacteria that were cultured overnight are still not expressing GFP and RFP
    • a colony of bacteria expressed RFP (to the naked eye) on a E0240 plate (GFP reporter)
      • most likely this is a labeling error

Trentay 14:26, 21 July 2009 (UTC)

Tasks

  • Make Cryogenic stocks of overnight cultures (2 vials were made of each one)
    • J23110 w/ E0240
    • J23110 w/ J06702
    • J23113 w/ E0240
    • J23113 w/ J06702
    • J23116 w/ E0240
    • J23116 w/ J06702
    • R0011 w/ E0240
    • R0011 w/ J06702
    • R0040 w/ E0240
    • R0040 w/ J06702
  • Dilute overnight cultures for flow cytometer tomorrow
    • 5mL was diluted with 1 mL of overnight culture
  • Organize notebook and running draft
  • Pick colonies from transformation of J23100 and J23106 with RET E0240

Trentay 14:26, 21 July 2009 (UTC)

Wetlab

  • Cryogenic stocks were made

Trentay 14:52, 21 July 2009 (UTC)

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