Virginia Commonwealth/21 May 2009

From 2009.igem.org

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Thursday 21 May 2009

Tasks

  1. Make stock of electrocompetent E. coli K-12
  2. Transform E. coli with the following:
    • BBa_I13521 (RPF PRET) - located in plate 2, well 6O AmpR
    • BBa_I13522 (GRP PRET) located in plate 2, well 8A AmpR
    • BBa_E0840 (GFP RET) - located in plate 1, well 12O AmpR
    • BBa_R0040 (Promoter) - located in plate 1, well 6I AmpR
    • pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in plate 1, well 13A KanR
    • BBa_E0240 (GFP RET) - located in plate 1, well 12M AmpR
    • BBa_J23101 (Promoter) - located in plate 1, well 18E AmpR
    • BBa_I20260 (Standard promoter) - located in plate 2, well 17F KanR
  3. Plate cells on LB with appropriate antibiotic - grow overnight

Results

  1. Plates were prepared for today's work.