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Virginia Commonwealth/2 July 2009
From 2009.igem.org
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Contents |
Thursday 2 July 2009
Results
Afton and Maria
- All replated (-) controls grew on both KAN plates (lots of little colonies)
- Troubleshooting this problem will be abandoned and new cells will be grown up and tested to ensure the protocols are being done correctly
- The one visible colony on the psB3k3 p1010 backbone with DB 3.1 have grown. It's still just one colony but it is much bigger.
- Overnight culture was successful
Trentay 18:48, 2 July 2009 (UTC)
Kevin and Adam
- parts J23101 and pSB3K3 showed no growth
- (+) controls for NEB10B and DB3.1 showed lawn growth
- (-) controls for NEB10B and DB3.1 showed no growth
- part J23119 showed < 10 colonies
- part E0840 showed hundreds of colonies
Tasks
Afton and Maria
- MiniPrep overnight culture
- Make stocks from overnight culture to freeze
- Begin planning out/documenting Project A in Microsoft Word.
- the goal is to keep a running draft
Trentay 18:48, 2 July 2009 (UTC)
Wetlab
Afton and Maria
- MiniPrep was done
- Cryogenic storage of DB 3.1 with backbone was frozen in the -80 degree Celsius freezer
Trentay 18:48, 2 July 2009 (UTC)
Kevin and Adam
- parts J23119 and E0840 were cultivated overnight from plate growths
- parts J23101 and pSB3K3 were cultivated overnight from cryogenic cell stock
Bussingkm 23:28, 2 July 2009 (UTC)
