Virginia Commonwealth/30 May 2009

From 2009.igem.org

May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

Saturday 30 May 2009

Results

  1. 15% glycerol stocks stored in -80, box C15.
  2. Miniprep completed as in the OWW protocol, doing two elutions of 20uL and 30uL to increase yield. Spec. used to assess yields:
Sample A260 A280 Ratio Conc. (ug/ml) Amount (ug)
J04450_1 0.082 0.050 1.640 123 ~5.6
J04450_2 0.077 0.047 1.638 115 ~5.3
I13521 0.082 0.049 1.673 123 ~5.6
  1. Plasmids were stored in -20 in box marked "BioBrick stocks"

Tasks

  1. Glycerol stocks and miniprep of plasmids from overnight cultures of J04450 and I13521.