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Virginia Commonwealth/4 June 2009
From 2009.igem.org
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Thursday 04 June 2009
Results
- Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - GMcArthurIV 15:03, 4 June 2009 (UTC)
Tasks
- Lab orientation and safety overview
- Synthetic biology overview discussion
Wetlab
- Transform NEB10β or DB3.1 with the following parts and plate overnight:
| Part No. | Type | ccdB gene | Resistance | Plate | Well | |
|---|---|---|---|---|---|---|
| R0040 | Promoter | No | AmpR | N/A | N/A | |
| E0840 | GFP RET | No | AmpR | N/A | N/A | |
| J13002 | Expression plasmid | No | AmpR | 1 | 13B | |
| E1010 | RFP CDS | No | KanR | 1 | 18F | |
| pSB1C3 | High-copy assembly plasmid | No | CmR | 1 | 5A | |
| pSB4C5 | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Controls (electroporate NEB10β or DB3.1 without plasmid DNA and grow on agar overnight):
| Part No. | Type | Resistant to ccdB | Resistance | |
|---|---|---|---|---|
| (+) Control | NEB10β on LB | No | None | |
| (-) Control | NEB10β on LB+Amp | No | None | |
| (-) Control | NEB10β on LB+Cm | No | None | |
| (-) Control | NEB10β on LB+Kan | No | None | |
| (-) Control | DB3.1 on LB+Cm | Yes | None | |
| (+) Control | DB3.1 on LB+Kan | Yes | None |
