Virginia Commonwealth/5 August 2009

From 2009.igem.org

May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

Contents

Wednesday 5 August 2009

Results

Maria and Afton

  • Transformation Results
Name Plate Growth Observation
pSB1C3 CM 5 small colonies
(+) Control LB lawn
(+) Control zapped LB lawn
(-) Control CM no growth

Trentay 13:19, 5 August 2009 (UTC)

Tasks

Maria and Afton

  • Pick colonies of pSB1C3 w/ P1010 for overnight culture
  • Make overnight culture of pSB4C5 and I1352 from frozen stocks
  • Work on Wiki

Trentay 13:19, 5 August 2009 (UTC)

Kevin & Adam

  • Digest and run gel on backbone
  • Digest synthetic promoters and ligate them into backbone with RET

Wetlab

Maria and Afton

  • Overnight 5mL culture of pSB1C3 w/ P1010
  • LB plates were made

Trentay 21:53, 5 August 2009 (UTC) Trentay 13:19, 5 August 2009 (UTC) Kevin

  • digested, ligated, and transformed promoter designs 5-9 with mCherry RET into pSB1C3 backbone.
Retrieved from "http://2009.igem.org/Virginia_Commonwealth/5_August_2009"