Virginia Commonwealth/6 July 2009

From 2009.igem.org

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Contents

Monday 06 July 2009

Results

  • n/a

Tasks

Maria and Afton

  • Gather information and choose parts for experiments (promoter, backbone, and RET)
  • Write running draft
  • Transfer information to new lab notebook

Trentay 19:36, 6 July 2009 (UTC)


Kevin and Adam

  • Continue research and further analyze and hone the list of DNA sequences to be synthesized


Craig and Clay

  • It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025). BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive.

Wetlab

Maria and Afton

  • n/a

Trentay 19:36, 6 July 2009 (UTC)