Virginia Commonwealth/6 June 2009

From 2009.igem.org

Virginia Commonwealth iGEM 2009

May
M	T	W	T	F	S	S
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31

June
M	T	W	T	F	S	S
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30

July
M	T	W	T	F	S	S
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31

August
M	T	W	T	F	S	S
					1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30
31

September
M	T	W	T	F	S	S
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30

October
M	T	W	T	F	S	S
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

Saturday 06 June 2009

Results

Note that undergraduates need key card access to the building.
All samples from yesterday's cultivation grew well.

Tasks

Harvested cells from cultivation
Purified plasmids using the mini-prep kit
Froze stock of cells carrying plasmids in box C-15

Wetlab

Mini-prep (plasmid purification) tutorial
- Data from plasmid purification


Part No.	A260 (nm)	A280 (nm)	A260/A280	Concentration (microgram/mililiter)	Amount (microgram)	Volume (microliter)
J13002	.026	.018	1.44	52	1.3	25
pSB1C3	.085	.050	1.700	170	9.69	57
R0040	.025	.017	1.471	50	2.000	40
E1010	.063	.038	1.658	126	4.536	36
pSB4C5	.101	.059	1.712	202	7.676	38
E0840	.084	.049	1.714	168	6.888	41

- Bussingkm 23:10, 6 June 2009 (UTC)

Note the Data recorded here was off by a factor of 10 for concentration and 1000 for amount and was edited on July 6th. MandM 15:28, 6 July 2009 (UTC)