From 2009.igem.org
Friday 7 August 2009
Results
- Overnight Culture of psB1C3, I1352, and pSB4C5 was successful.
- I1352 showed pink florescence
Trentay 23:49, 8 August 2009 (UTC)
Tasks
- Plan for the next few Days
| Friday | Monday | Tuesday | Wednesday
|
| Miniprep pSB1C3 w/ P1010 | Digest 5-9, pSB1C3, J06702 | Pick colonies | Make Stocks
|
| Digest J23100/110, pSB1C3, J06702 | Run a gel | - | -
|
| Run a gel | Ligate parts | - | -
|
| Ligate parts | Transform parts | - | -
|
| Transform parts | - | - | -
|
Trentay 20:58, 6 August 2009 (UTC)
Maria and Afton
- Make Cryogenic stocks of overnight cultures (2 vials each)
- Miniprep two vials of pSB1C3 w/ P1010, and one vial of I1352 and pSB4C5
- Run a Gel on the Miniprepped DNA
- Gels are run at 100 volts for 1 hour
- Take Spectrophotometry measurements
- Digest parts
- Run a Gel on Digested DNA
- Ligate pSB1C3 with I1352 and I1352 with itself
- Transform ligations into NEB10β
- This was done, because if the Death gene P1010 went back into the 1C3 backbone, the cell would die, so only desirable parts would grown on an LB plate
Trentay 23:49, 8 August 2009 (UTC)
Wetlab
- 1 Cell stock of parts BBa_I13600, BBa_K118024, BBa_K118025, BBa_I742111 was made and stored in 15% glycerol in the -80C freezer.
- 2 The same parts were minipreped using the standard protocol
-Bussingkm 22:42, 7 August 2009 (UTC)
Maria and Afton
- Cryogenic stocks were made
- Miniprep was done
- Gel was run for miniprepped data
- Digest was done
- Gel was run for digested data
- Gel will need to be redone because the wrong ladder was used, so bands could not be compared to the ladder
- Ligations were done
- Transformations were done
Trentay 23:57, 8 August 2009 (UTC)
|
"
