Virginia Commonwealth/8 July 2009

From 2009.igem.org

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Contents

Wednesday 8 July 2009

Results

Afton and Maria

  • Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test
    • Possibly pSB3K3 in the iGem stock is a bad part
  • Gel Electrophoresis Result
from left to right: pSB1C3,1kB ladder,pSB4C5
  • Miniprep Results:
Part No. A260 (nm) A280 (nm) A260/A280 Vol. (µL) Conc. (µg/mL) Amt (µg)
pSB4C5 0.051 0.032 1.594 102 47 4.79
pSB1C3 0.049 0.029 1.690 98 34 3.33

Trentay 18:50, 8 July 2009 (UTC)

Tasks

Afton and Maria

  • Digestion of backbones: pSB1C3, pSB4C5
  • Spectrometry of backbone miniprepped stocks
  • Transform promoter 106, RET mCherry when cuvettes arrive

Trentay 16:49, 8 July 2009 (UTC)

Wetlab

Afton and Maria

  • Digestion
  • Agarose Gel preparation/Electrophoresis test
  • Parts (J06702,J23106) were taken from iGem wells, electrotransformed, and plated.

Trentay 21:26, 8 July 2009 (UTC)

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