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Wisconsin-Madison/10 July 2009
From 2009.igem.org
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July 10, 2009
Ex 20: New Cloning Methods
Inoculate DH1 (Blank – No resistance)
Redone Digestion from Yesterday:
Left to right: NudF 1b undigested-digested- NuF2B undigested-digested- Ladder- choline 1 undigested - digested- backbone undigested- digested
1. NudF – (560+2157=) 2717 bp
2. Choline – (2000+2157=) 4157 bp
3. Backbone P1 – 2157+1069 bp
4. (pVP68K): gsSDMT (Pst1) – (931+5212=) 6143 pb
Ex 10: Cyanobacteria
1. Spray everything with 70%
2. Add 9 mL Media A to cultures (7/9/09)
3. Add 7.35 uL CM to cultures (7/9/09)
4. Shake - 30C Cyano Room
Ex 19: Parts from Registry
Inoculate 1.O22
Other:
Research
1st Antiporter gene has cut with Pst1
2nd Antipoter gene has no iGEM cut sites
Primer Design:
1st Antiporter finalized
2nd Antiporter finalized
New ProU finalized
Primers Ordered: MevT, MetK, ProU again, Gssdmt, Chris's two antiporter gene
Ex 18: pTaq
Made two protein gels
Ran Taq Protein
Stained protein gel
Write our Meeting Notes
ProU, ProV cutting and cloning
