Wisconsin-Madison/10 June 2009

From 2009.igem.org

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June 10, 2009

Other: Set up a little bit of the Cyano growing room on the third floor

Innoculation of Cyano cultures (Cyano room)

Received spinach cDNA

Cleaned and autoclaved glasswear


Ex 9: MiniPrep: of MetK gene

Ran Gel of non-digested DNA (pic)

Made Glycerol stock of DNA

Waiting on Dan to send sequence to do Restriction Digest

Gel 0610.jpg


Ex 10: Cyanobacteria

Made freezer stocks of MetK and streaked plates of pSB1A3, pRL1383A, and pMMBRraA to remake freezer stocks - NP

Inoculated first culture of Synechococcus elongatus PCC 7942 (5:1, BG11:culture ratio) - NP