Wisconsin-Madison/15 October 2009

From 2009.igem.org

Calendar

April
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6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30

May
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June
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29	30

July
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August
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31

September
M	T	W	T	F	S	S
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28	29	30

October
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19	20	21	22	23	24	25
26	27	28	29	30	31

October 15, 2009

Cloning

- Digestion

- PCR Clean up

- Ran Gel

- Found concentrations

- Ligations

PhotoPromoter: pHawaii

pHawaii - vector only

ProtoPromoter: pSB1A2

pSB1A2 - vector only

ProU:RBS+RFP

RBS+RFP - vector only

Thermocycler -> overnight

Inoculations

- 5 confirmed BioBricks

- New Parts

- Genes in pBAD for testing in Minimal Media

Sending DNA

- Prepared DNA in single PCR Tubes

Retrieved from "http://2009.igem.org/Wisconsin-Madison/15_October_2009"