Wisconsin-Madison/19 May 2009
From 2009.igem.org
Calendar
|
|
|
|
|
|
|
May 19, 2009
Chemical Safety Training: [ChemTraining]
Biological Satety Training: [BioTraining]
Ex 1: YhfR grew on Amp resistance plate
NudF grew on Amp resistance plate
Made liquid cultures of NudF and YhfR
Ex 2: Plated plasmids obtained from Dan;
1. pBAD33 –Cm resistance
2. pTrc 99A – Amp resistance
3. pBt2BAD18 – Kan resistance
- inoculated pNudF and pYhfR liquid cultures in LB media for overnight growth - NP
- obtained plasmids pBAD33 (cm), pTrc99A (amp), and pBT2bad18 (kan) from Dan Agnew and grew on appropriate plates overnight - NP