Wisconsin-Madison/20 May 2009

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May 20, 2009

Ex 2: Growth on following plates

1. pBAD33 –Cm resistance

2. pTrc 99A – Amp resistance

3. pBt2BAD18 – Kan resistance

Made liquid cultures of the three plasmids


Other:

Made antibiotics: Amp, Cm, Kan

Research


Ex 1: MiniPrep of NudF and YhfR acroding to miniprep protocol

Results: out of RNAase and got bad results from our gel

Gel 05-20.jpg


- mini preparation of pNudF and pYhfR according to the mini prep protocol of Scott Walhovd, bad results b/c no RNAase - NP

- inoculated liquid cultures of Dan's plasmids from yesterday in LB media with appropriate antibiotics - NP

- made freezer stocks of pNudF and pYhfR - NP

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