"
Wisconsin-Madison/23 June 2009
From 2009.igem.org
Calendar
|
|
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
June 23, 2009
Ex 10: Set up fish tank with bubbler of CO2
12 hour timer – or else cyanos will photo-bleach with too much light
Ex: Miniprep of
1. gsSDMT
2. (pSBA1) ProU BB
3. (pSBA1) ProU, GFP BB
Digestion:
Digested(proUBB, GFP+ProUBB, proU, GFP+ProU) Ladder undigested (proU, GFP+ProU)
NO IDEA WHAT THIS MIGHT MEAN!
ProU, GFP BB
Might get better readings with smaller load – could be super concentrated
Ex 18: pTaq induction
Ex 17: GFP Regulation by ProU Promoter
Test 1:
Test of GFP and Pro Biobrick
Conc. / Culture (uL) / 5M NaCl (uL)
0.0 / 200 / -
0.2 / 192 / 8
0.4 / 184 / 16
0.6 / 176 / 24
0.8 / 168 / 32
1.0 / 160 / 40
Well Assignment:
Conc. / Wells
0.0 / D5 / E8 / C6 / F9
0.2 / E5 / D8 / F6 / C5
0.4 / D6 / F4 / C8 / F8
0.6 / E9 / D5 / C7 / F7
0.8 / D9 / E4 / D7 / C4
1.0 / C9 / F5 / E6 / E8
All other wells were filled with MilliQ water
Ex 10: Cyanobacteria
Inoculated two cultures of PCC 7942 to test the new aeration system (one normal flow, one restricted flow) - NP
-tested freezer stock of PCC 7942 by plating on BG11 (used entire 1mL stock) check ~June 30 for results (plates dehydrated so unsuccessful) – NP
