Wisconsin-Madison/28 May 2009

From 2009.igem.org

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May 28, 2009

Other: Meeting with Brian


Ex 4:

MiniPrep of pMevT, MpBI, pMBIS

Ran gel

Made freezer stock

Gel0528.jpg

Spec DNA to determine concentration:


Ex 5: Ecoli Transformations:

1. Double transformation of NudF and MevT …plated on Cm, Amp

2. Triple transformation of NudF , MevT, and MBI… plated on Cm, Amp, Tet

3. control: Double transformation of pBAD33 and pTrc99A plated on Cm, Amp


- mini preparation of pMevT, pMBIS, pMBI - NP

- made freezer stocks of pMevT, pMBIS, pMBI - NP

- tested the concentration of the DNA with a spectrophotometer - NP

- ran a gel of pMevT, pMBIS, pMBI (successful) - NP

- transformed competent cells with pMevT, pMBI, and pNudF (all three in one and another with pMevT and pNudF only, only the double was successful) - NP