Wisconsin-Madison/28 May 2009
From 2009.igem.org
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May 28, 2009
Other: Meeting with Brian
Ex 4:
MiniPrep of pMevT, MpBI, pMBIS
Ran gel
Made freezer stock
Spec DNA to determine concentration:
Ex 5: Ecoli Transformations:
1. Double transformation of NudF and MevT …plated on Cm, Amp
2. Triple transformation of NudF , MevT, and MBI… plated on Cm, Amp, Tet
3. control: Double transformation of pBAD33 and pTrc99A plated on Cm, Amp
- mini preparation of pMevT, pMBIS, pMBI - NP
- made freezer stocks of pMevT, pMBIS, pMBI - NP
- tested the concentration of the DNA with a spectrophotometer - NP
- ran a gel of pMevT, pMBIS, pMBI (successful) - NP
- transformed competent cells with pMevT, pMBI, and pNudF (all three in one and another with pMevT and pNudF only, only the double was successful) - NP