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Wisconsin-Madison/2 July 2009
From 2009.igem.org
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July 2, 2009
Other: Brian Meeting
Research
Proposal for Genes Abroad
Ting took her MCAT!
Ex 15: Inducing Various Modified (triple) E.Coli Strains
2. MG1655 W: Trip – GROWTH ON PLATE – SUPPOSED TO DIE FROM TOXICITY OF INTERMEDIATES!
SO MUCH HERE IS SO SO WRONG – ran out of Triple plates
1. Control 33+99: Cm+Amp - EH
2. MG1655 W: Trip - Maybe some type of Isoprene
3. MG1655 Delta Ara: Trip – Contamination of Sample - could not analyze
4. BL21 (DE3): Trip - Maybe some type of Isoprene
NO ISPOPENTENOL - See meeting for game plan
Primers: Primer Design for MevT and MetK
Do not need primers for Photosystem Promoter – having it sequenced
Ex 18: pTaq Induction and experiments
MiniPrep of pTaq
Ex 10: Cyanobacteria
Tested freezer stocks of 7942 (25% glyc and 50:1 centrifugation/resuspention) again on BG-11 plates with appropriate plating (parafilm wrap) - NP
